🔹 Product Overview

PNC-27 10mg is a synthetic 27–amino acid research peptide derived from the third alpha-helical segment of the tumor suppressor protein p53 (ASPP2 region). It has been studied in laboratory and preclinical models for its potential to selectively interact with cancer cell membranes via binding to human double minute 2 (HDM-2) protein.

Important: PNC-27 10mg is supplied strictly for laboratory research use and is not approved for human or animal use.

🔹 What Is PNC-27 10mg?

PNC-27 10mg is an experimental peptide originally synthesized around the year 2000 during research associated with immunodeficiency studies. Subsequent studies identified its ability to bind HDM-2, a protein overexpressed on the plasma membranes of various cancer cell types.

Unlike many anticancer research compounds that induce apoptosis, PNC-27 has been studied for its ability to induce necrotic cell death through direct membrane disruption in experimental models.

PNC-27 10mg remains strictly for controlled scientific investigation.

🔹 Research Background

• Research has focused on PNC-27’s selectivity toward cancer cells in lab studies.

• HDM-2 protein localizes on membranes of certain cancer cells but not normal cells.

• This led to studies on whether PNC-27 could selectively bind cancer cell membranes while sparing normal cells.

In vitro studies include cancer cell lines of:

• Pancreatic cancer

• Ovarian cancer

• Leukemia

• Breast tumor models

⚠️ Findings are preclinical and not validated in human clinical trials.

🔹 Mechanisms of Research Interest

HDM-2 Binding and Selective Targeting

• PNC-27 10mg forms a 1:1 complex with membrane-bound HDM-2, initiating downstream membrane-disruptive events.

Transmembrane Pore Formation

• PNC-27 may participate in forming transmembrane pores, perforating cancer cell membranes.

• This process allows ion influx and intracellular efflux, known as membranolysis.

Necrotic Cell Death Research

• Induces necrosis instead of apoptosis.

• Monitored via lactate dehydrogenase release, a marker of membrane disruption and cell lysis.

Peptide Integrity and Activity

• Intact peptide is required for activity.

• Peptide fragments do not produce membranolytic effects, highlighting structural integrity importance.

Cancer Cell Selectivity

• Immuno-electron microscopy shows ring-shaped pore structures on cancer cells exposed to PNC-27.

• Structures absent in normal cells unless HDM-2 protein artificially introduced, supporting selective activity.

⚠️ All mechanisms are experimental or theoretical. No therapeutic benefit implied.

🔹 Molecular and Chemical Information

• Product Name: PNC-27 10mg

• Peptide Length: 27 amino acids

• Origin: p53 (ASPP2 alpha-helical segment)

• Form: Lyophilized powder

• Purity: Research-grade (>99%)

🔹 Research Applications

PNC-27 10mg may be used in laboratory investigations involving:

• Cancer cell membrane biology research

• HDM-2 protein interaction studies

• Necrotic cell death pathway research

• Membrane pore formation and cytolysis models

🔹 All use must remain within controlled laboratory environments.

🔹 Regulatory Status and Disclaimer

• For research use only

• Not approved by FDA

• Not for human or veterinary use

• Not intended to diagnose, treat, cure, or prevent any disease

• No established clinical safety, dosing, or efficacy data